| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190189 |
| Chromosome: | chromosome 4 |
| Location: | 1189450 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215550 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAGGTTGATGCAGCCCAGTTTGTGGT |
| Internal bar code: | AATAATGGAATAACGGGCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 96.4281 |
| LEAP-Seq n confirming: | 7532 |
| LEAP-Seq n nonconfirming: | 279 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCCGTATTCATCCGCTT |
| Suggested primer 2: | CACACACACTCGTGTTTCCC |