Insertion junction: LMJ.RY0402.190207_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.190207
Chromosome:scaffold 24
Location:63661
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre24.g755597 Chlorophyll a /Pheophorbide a oxygenase-related protein; (1 of 2) 1.14.13.122 - Chlorophyllide-a oxygenase / Cholorophyll-b synthase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACGCTGGGCGCGGGTGAGTTTGTGGGG
Internal bar code:CGAGGGACCGACTTTAGGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:96.4444
LEAP-Seq n confirming:1953
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTCACTCCACTCCATTCC
Suggested primer 2:AAACTGTATTGGACGACGGC