| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190208 |
| Chromosome: | chromosome 2 |
| Location: | 2720623 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093650 | (1 of 1) 1.14.12.22 - Carbazole 1,9a-dioxygenase / CARDO | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGCCAAGTTGCAAGTCAGGACTCAGGA |
| Internal bar code: | ATGCGGCCAGCGATGGCCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCATGGCAGTGAATCTT |
| Suggested primer 2: | CTGCCGAGAAAGCATACACA |