| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190226 |
| Chromosome: | chromosome 1 |
| Location: | 3955615 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025750 | (1 of 1) PF01551 - Peptidase family M23 (Peptidase_M23) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGGACGGGCGGCCCAAGTCTTCCAGAC |
| Internal bar code: | CGCCGCCCTGCACAGAAGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 98.2393 |
| LEAP-Seq n confirming: | 2176 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTTGTGTCGGAGTTGT |
| Suggested primer 2: | GGCACATCCGTAGTCAGGTT |