| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190236 |
| Chromosome: | chromosome 1 |
| Location: | 4407490 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029900 | (1 of 3) 3.6.3.17 - Monosaccharide-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAGTCAGAGGAGAGCGGGGTAGAGGTG |
| Internal bar code: | CAAAAGTTTCATTATTTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 99.8476 |
| LEAP-Seq n confirming: | 655 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGGTCGCAACCAAAAAC |
| Suggested primer 2: | AGTTTACCCAACCCTCGCTT |