| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190249 |
| Chromosome: | chromosome 4 |
| Location: | 2121923 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219500 | DNJ30 | (1 of 1) PTHR22904:SF338 - LD24721P; DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCTTTCCCTGCCACGTGTACCGTGTA |
| Internal bar code: | CACGTGAGTTTTCGTAGGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1268 |
| LEAP-Seq percent confirming: | 99.4827 |
| LEAP-Seq n confirming: | 2500 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTCTTGTGGGGTGGAGG |
| Suggested primer 2: | ATCATTATGCGGTGCTAGGG |