| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190267 |
| Chromosome: | chromosome_7 |
| Location: | 1943822 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGGTCTCGGTGGTTTGGGAGCACTTCTG |
| Internal bar code: | GGGTGAGCTTGGCGGTTAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 97.8283 |
| LEAP-Seq n confirming: | 946 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGCATAGCGGTCGTTT |
| Suggested primer 2: | TCAAACAAGCCAGTGTCGTC |