| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190298 |
| Chromosome: | chromosome 12 |
| Location: | 6079676 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535550 | (1 of 1) PTHR10992//PTHR10992:SF834 - ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN // ALPHA/BETA-HYDROLASE-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCGGGCAACGGCGAGTCCGAGGTGCGG |
| Internal bar code: | GGGCGCCAAAAGAACGACTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 97.4998 |
| LEAP-Seq n confirming: | 16106 |
| LEAP-Seq n nonconfirming: | 413 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAGCTCTTGATTTCGGC |
| Suggested primer 2: | AGGCTTCGTTCTGCTGTTGT |