Insertion junction: LMJ.RY0402.190356_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.190356
Chromosome:chromosome 4
Location:3757677
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g229494 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGTGCAGAAGTCTTTTCGCGAGTATTGT
Internal bar code:GTCACCTCACCTGGGCGGCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:469
LEAP-Seq percent confirming:99.7522
LEAP-Seq n confirming:805
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGTATGCATCGAACACAGCA
Suggested primer 2:GAGAACACAGGAGGAGTCGC