Insertion junction: LMJ.RY0402.190370_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.190370
Chromosome:chromosome 2
Location:6927220
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g119950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGCGGTAAAGGCACAGACAGGGCGCGC
Internal bar code:TTGAGGGTGTCTGATCTCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:744
LEAP-Seq percent confirming:34.1667
LEAP-Seq n confirming:287
LEAP-Seq n nonconfirming:553
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAACAATTGCATGGCGTT
Suggested primer 2:CACATTATTGGTCGCCTGTG