Insertion junction: LMJ.RY0402.190375_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.190375
Chromosome:chromosome 2
Location:2284482
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g090451 (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGCGCCTTGCGCACCCACCTCGCCAGC
Internal bar code:TTACGCTTGGCTGGTATCTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:850
LEAP-Seq percent confirming:99.3159
LEAP-Seq n confirming:2323
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCTTGCATCCAATACAAA
Suggested primer 2:TGGCAAGGAGAAAAGAGGAA