Insertion junction: LMJ.RY0402.190395_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.190395
Chromosome:chromosome 13
Location:3135644
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g585050 (1 of 3) K17285 - selenium-binding protein 1 (SELENBP1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATAGTAGGTGGCGACGGCGCCCATCTTCT
Internal bar code:CGCAACCGTGTGAAGCTAGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:591
LEAP-Seq percent confirming:95.7871
LEAP-Seq n confirming:19417
LEAP-Seq n nonconfirming:854
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTCATCTGTACCCCCTA
Suggested primer 2:ACAGTGGAAGTGTCTGTGCG