| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190396 |
| Chromosome: | chromosome 9 |
| Location: | 4496678 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395769 | FAP177 | Flagellar Associated Protein 177 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCGACGCGACGGTGGCTCCTCAAGTA |
| Internal bar code: | CTCCGGATGCCGGTTGGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 99.4516 |
| LEAP-Seq n confirming: | 544 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGCTAACAAGGTGGATT |
| Suggested primer 2: | ACTGACGTCACACTACCCCC |