| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190401 |
| Chromosome: | chromosome 8 |
| Location: | 4846998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384600 | (1 of 2) 3.1.1.81 - Quorum-quenching N-acyl-homoserine lactonase / Quorum-quenching N-acyl homoserine lactone hydrolase | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAAACGCTACAATTAAGGCACTTTCGG |
| Internal bar code: | AGTTTCCCAGTCACGAATCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 99.5868 |
| LEAP-Seq n confirming: | 5785 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGCTCTTGTGATGCAA |
| Suggested primer 2: | CATCTGGTGTAACGTCGTGG |