| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190439 |
| Chromosome: | chromosome 6 |
| Location: | 8651428 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309450 | CPZ | (1 of 1) 3.4.17.12 - Carboxypeptidase M | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGCCAAACTCAGTGTGTTTTACCACATA |
| Internal bar code: | CCCGTACACCAAGCCGCCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 12 |
| LEAP-Seq percent confirming: | 98.4496 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATGAGGTTTTAGCAGGA |
| Suggested primer 2: | CCAGTGAATCAGACAGGGGT |