| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190440 |
| Chromosome: | chromosome 16 |
| Location: | 431785 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693202 | SIR1 | Ferredoxin-sulfite reductase; (1 of 2) 1.8.7.1 - Assimilatory sulfite reductase (ferredoxin) / Sulfite reductase (ferredoxin) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCATCACCGAGGACTCGGTGCAGCTGA |
| Internal bar code: | ATTTTGGTGAAGCTGAAACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 99.2233 |
| LEAP-Seq n confirming: | 511 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGGGAGGAGAATGAAG |
| Suggested primer 2: | CTTGATGACGGAGCTGAACA |