| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190448 |
| Chromosome: | chromosome 17 |
| Location: | 762451 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701450 | (1 of 12) PTHR13078:SF22 - MAOC-LIKE DEHYDRATASE DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCTAGCCGGCGGCAGCGGGCAAACCTG |
| Internal bar code: | GTGATCCGAAGCCAGTCGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 99.5958 |
| LEAP-Seq n confirming: | 6160 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTTTGGTTGCATCGGTA |
| Suggested primer 2: | GCTGCTAAAGGCCAGTTCC |