| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190454 |
| Chromosome: | chromosome 17 |
| Location: | 5613155 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738050 | AGG4 | Aggregation 4; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGAGAGCTGATGACCCTTCTGAACGTC |
| Internal bar code: | GGTTTCGTAAATAGGGCCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 99.1597 |
| LEAP-Seq n confirming: | 2124 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGTGGCAATCAGGAAGC |
| Suggested primer 2: | ACATGATGGGATGGAACGAT |