| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190482 |
| Chromosome: | chromosome 3 |
| Location: | 729047 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146087 | (1 of 3) 1.14.11.33 - DNA oxidative demethylase / Alkylated DNA repair protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCATCACCAGCAGCCTCACAGAAGCC |
| Internal bar code: | GCGGATTTACCTGAGGCCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 24.1125 |
| LEAP-Seq n confirming: | 1277 |
| LEAP-Seq n nonconfirming: | 4019 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCCCTGCAGACTACAAC |
| Suggested primer 2: | TCGATATAAGCATGGGGAGC |