| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190514 |
| Chromosome: | chromosome 5 |
| Location: | 3342743 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240650 | DNJ32 | (1 of 1) K09506 - DnaJ homolog subfamily A member 5 (DNAJA5); DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGGGTCATAAGGCTACCGGTATCTCAA |
| Internal bar code: | GCGTCGACAAAATGGTCTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.3078 |
| LEAP-Seq n confirming: | 6169 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGGTGCTGAAGAAGGAG |
| Suggested primer 2: | ATTAAACAAGTTCGCACGGG |