| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190518 |
| Chromosome: | chromosome 3 |
| Location: | 8588987 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202673 | CSC2 | Chlamydomonadale-specific family C protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCAAACCAGCAGCCTGCACCCGCACAT |
| Internal bar code: | AGTGAGGACACGGAACCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.3421 |
| LEAP-Seq n confirming: | 604 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACCAACTCCTGTTGGGA |
| Suggested primer 2: | AGCCTTTAAGCTACCTCCGC |