| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190565 |
| Chromosome: | chromosome 10 |
| Location: | 2045150 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432900 | AKR3 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF170 - ALDO/KETO REDUCTASE // ALDOSE REDUCTASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCCACGCTGTGCTGCCTGTCCCCCTTT |
| Internal bar code: | TACCTCGCGTAGAGTATCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 99.7065 |
| LEAP-Seq n confirming: | 1019 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCACAAACCCACGACTT |
| Suggested primer 2: | GAGAACCTGGAGGGCATGTA |