Insertion junction: LMJ.RY0402.190581_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.190581
Chromosome:chromosome 5
Location:1044983
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g246300 (1 of 1) PTHR13554//PTHR13554:SF10 - 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED // 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGAAAGAGAGCAGTGACGGGCTTGTGA
Internal bar code:GCCGATCTTACGGGGTTTTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:49
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTAGGATACAAAGCCCG
Suggested primer 2:AACACAGCAGCGAATGACAC