| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190618 |
| Chromosome: | chromosome 3 |
| Location: | 3699300 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169300 | (1 of 2) K05681 - ATP-binding cassette, subfamily G (WHITE), member 2 (ABCG2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGGCTCACGGCAGTGATGGGGGCGTC |
| Internal bar code: | CGGCTGTTAGTGGGTGAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 98.3969 |
| LEAP-Seq n confirming: | 1289 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCTGTCAACAATCCCTC |
| Suggested primer 2: | ACACTGTCCAGCGACTTGTG |