| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190664 |
| Chromosome: | chromosome 7 |
| Location: | 4210275 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342100 | (1 of 1) IPR004046//IPR010987//IPR012336 - Glutathione S-transferase, C-terminal // Glutathione S-transferase, C-terminal-like // Thioredoxin-like fold; Glutathione S-transferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATTTCAGTCAGGTGTACACAGGTGGCAC |
| Internal bar code: | GTCGGCGGACGTGGAGGTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1172 |
| LEAP-Seq percent confirming: | 99.4161 |
| LEAP-Seq n confirming: | 681 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGTATGCAATGTGAACC |
| Suggested primer 2: | AGATGGTGGACGCACACATA |