| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190665 |
| Chromosome: | chromosome 4 |
| Location: | 704146 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217954 | (1 of 1) K05850 - Ca2+ transporting ATPase, plasma membrane (ATP2B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGTGACTCCTAAAGGTCTGTAGTAGTT |
| Internal bar code: | GCAGTCGGGCTCGGAACTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 99.6403 |
| LEAP-Seq n confirming: | 277 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGCGTTACAATCCGCC |
| Suggested primer 2: | CAAACTTTAGGGCAACCCAA |