| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190702 |
| Chromosome: | chromosome 10 |
| Location: | 1949646 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432250 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCAACTGTGCCGGACCTAACATACTGT |
| Internal bar code: | TCGCGTACGCATGACTCGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 98.6607 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACCATGTGTTTGACCA |
| Suggested primer 2: | CAGGTTCTTTTTGCACAGCA |