| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190709 |
| Chromosome: | chromosome 6 |
| Location: | 6874179 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295500 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAACTTGGCGGGGATGAAGGGGATTGGC |
| Internal bar code: | TCAAGGAGGCGACCTGCGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 98.2281 |
| LEAP-Seq n confirming: | 2162 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGAGCCCATACGTACACA |
| Suggested primer 2: | TGTGTCTGGCCTCTGTTCTG |