Insertion junction: LMJ.RY0402.190728_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.190728
Chromosome:chromosome 10
Location:4355502
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g451250 (1 of 3) PTHR13620:SF0 - EXONUCLEASE 3'-5' DOMAIN-CONTAINING PROTEIN 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGGCCGCCTGCAGTAGCTGCCCACTCT
Internal bar code:CGCGAGGAGCTGGAGGCTAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:77.2425
LEAP-Seq n confirming:465
LEAP-Seq n nonconfirming:137
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCGCTCCACAAGTGAATA
Suggested primer 2:TTCCCTTGTCTCATGGAAGG