| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190736 |
| Chromosome: | chromosome 17 |
| Location: | 5071021 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734805 | (1 of 8) PF00560 - Leucine Rich Repeat (LRR_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACCAAATAAACTCAAACCCGGCAGCGTT |
| Internal bar code: | TAAAGATGTGGAACACAGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 99.877 |
| LEAP-Seq n confirming: | 812 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACTGGCATCGTTACGGG |
| Suggested primer 2: | GAGCCATTGGTGAACGTTTT |