| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190747 |
| Chromosome: | chromosome 6 |
| Location: | 1135917 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257050 | GBS2 | Granule bound starch synthase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAACCGCGGATGCCACGCAGAGCGCAGC |
| Internal bar code: | GCTGGGAACCCCGCGCGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 803 |
| LEAP-Seq percent confirming: | 99.6971 |
| LEAP-Seq n confirming: | 5267 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGATGTCTCCTGCTCC |
| Suggested primer 2: | GGCATGTTGATTGTTGAACG |