| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190768 |
| Chromosome: | chromosome 6 |
| Location: | 6562766 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293516 | DEGO2 | (1 of 4) PTHR22939:SF83 - PROTEASE DO-LIKE 2, CHLOROPLASTIC; Inactive Deg protease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGCGGTCCATGGCGGGACCGGCGTCC |
| Internal bar code: | GGCGCGTACCACGCGGGCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 99.7727 |
| LEAP-Seq n confirming: | 439 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGACTGCTGTCGTTGA |
| Suggested primer 2: | CTCTCCTTCCTCCTCCTCGT |