| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190777 |
| Chromosome: | chromosome 12 |
| Location: | 4604589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522650 | (1 of 2) 2.4.1.232 - Initiation-specific alpha-1,6-mannosyltransferase / Glycolipid 6-alpha-mannosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGACGGACCGGGCGCACCCCGAGGTGG |
| Internal bar code: | CTCAGTTCTGTCGGGCTGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 999 |
| LEAP-Seq percent confirming: | 99.2461 |
| LEAP-Seq n confirming: | 8557 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCTACAATGTCAGCCT |
| Suggested primer 2: | ACACCATACACGACGCAAAA |