Insertion junction: LMJ.RY0402.190783_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.190783
Chromosome:chromosome 6
Location:4326052
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278260 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACCAGCTCTGCAGCCCCTCCCCTATCA
Internal bar code:CAGCCCCACACTCCCCATACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:189
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:121
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGACACCATGCACAA
Suggested primer 2:AAGGAACAGGGCAGGTTAGC