Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.190784 |
Chromosome: | chromosome_9 |
Location: | 2138553 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g393450 | FAP233 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCTACGTCATCAGCCAGGTGCTGTTCCCA |
Internal bar code: | TACTAGACAACACCGTGTTGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1052 |
LEAP-Seq percent confirming: | 99.5934 |
LEAP-Seq n confirming: | 22047 |
LEAP-Seq n nonconfirming: | 90 |
LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACATCCTCATCGGCAACAA |
Suggested primer 2: | TTATGCTTGCATGTGGGTGT |