| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190799 |
| Chromosome: | chromosome 1 |
| Location: | 4859606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGGCACGGCGTCATCCAGAGGGCCGAC |
| Internal bar code: | TCAATTTGAATGTCTTGTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 88.7324 |
| LEAP-Seq n confirming: | 189 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGGTCAGCTGCATGTC |
| Suggested primer 2: | ACCCACACCCTCTCTCACAC |