| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190818 |
| Chromosome: | chromosome 2 |
| Location: | 6147323 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113500 | (1 of 1) IPR001322//IPR014867 - Lamin Tail Domain // Spore coat protein CotH | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTGTACAGGAGCTCCCCGTCTGCGTG |
| Internal bar code: | CGGCCACTGGGTCGGCTCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 96.1637 |
| LEAP-Seq n confirming: | 376 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACAATCTGACCACTTCCC |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |