| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190821 |
| Chromosome: | chromosome 12 |
| Location: | 8453596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548250 | (1 of 1) K13216 - nuclear inhibitor of protein phosphatase 1 [EC:3.1.4.-] (PPP1R8, NIPP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTATGCCCCATGCGCAACTAGAGCCATGG |
| Internal bar code: | ATAGGGCGCGCACGTTGTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 98.8649 |
| LEAP-Seq n confirming: | 871 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCACACTTCAGTCCTACA |
| Suggested primer 2: | GGATACGTAAGGGTACGGCA |