| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190828 |
| Chromosome: | chromosome 2 |
| Location: | 5706336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110050 | (1 of 1) PTHR22844:SF160 - WD40 REPEAT-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGTAGCAGCCAGCAGCACGCATCAAG |
| Internal bar code: | ACAGCACCAATAACGCGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 106 |
| LEAP-Seq percent confirming: | 74.5763 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTTCAGTGACTCCAGG |
| Suggested primer 2: | GCAGGCAAGAGGTTGAAGAC |