| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190828 |
| Chromosome: | chromosome 2 |
| Location: | 5706343 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110050 | (1 of 1) PTHR22844:SF160 - WD40 REPEAT-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTGTCACGCGCCAACAGCCGCTGCGAC |
| Internal bar code: | TTATAATCTTCAGTCTTCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 98.991 |
| LEAP-Seq n confirming: | 2845 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTTCAGTGACTCCAGG |
| Suggested primer 2: | GCAGGCAAGAGGTTGAAGAC |