| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190851 |
| Chromosome: | chromosome 3 |
| Location: | 7700984 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203700 | DEGO1,DEG12 | DegP-type protease-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCCCGTGCATGTGTATCACGGCGTGGC |
| Internal bar code: | GCAACCAGGCCCAGTTTACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 24 |
| LEAP-Seq percent confirming: | 74.8373 |
| LEAP-Seq n confirming: | 1035 |
| LEAP-Seq n nonconfirming: | 348 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTTTGGCTGGGAAGAAA |
| Suggested primer 2: | GGTGACCAAGGAAAAGGACA |