| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.190867 |
| Chromosome: | chromosome 13 |
| Location: | 1359395 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571560 | (1 of 1) PF09737 - De-etiolated protein 1 Det1 (Det1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGGAACTGGAGAAGAGAGAATGGCGGG |
| Internal bar code: | ACCCCCACGTAAAGCTCGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 372 |
| LEAP-Seq percent confirming: | 61.5826 |
| LEAP-Seq n confirming: | 537 |
| LEAP-Seq n nonconfirming: | 335 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGATGCTGTTTGACCTGC |
| Suggested primer 2: | CACACACACACACACACGGT |