Insertion junction: LMJ.RY0402.190869_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.190869
Chromosome:chromosome 17
Location:668048
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g700700 (1 of 37) IPR008979 - Galactose-binding domain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTGGCACTGCACCGTAGCCGCACATCG
Internal bar code:GCGAGTATGAATATTCAAGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:227
LEAP-Seq percent confirming:99.6377
LEAP-Seq n confirming:275
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGTTCTGGAGCACGACA
Suggested primer 2:TCAGGACGTCATAGCGACTG