| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190897 |
| Chromosome: | chromosome 1 |
| Location: | 3680464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023650 | Probable amino acid/metabolite permease; (1 of 4) PTHR11785:SF367 - AMINO-ACID PERMEASE BAT1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCTGCTGGATGACGGCGTGGATCCTCA |
| Internal bar code: | CGAGGTCGCGCTTTCCGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 99.5985 |
| LEAP-Seq n confirming: | 5706 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACATAGCTGGACCCCTC |
| Suggested primer 2: | TCAATCCCAAAGAACCAAGG |