| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.190898 |
| Chromosome: | chromosome 6 |
| Location: | 7653222 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301550 | MFT18 | Major facilitator superfamily transporter; (1 of 3) PTHR23505//PTHR23505:SF21 - FAMILY NOT NAMED // SPHINGOLIPID TRANSPORTER SPINSTER HOMOLOG 1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGGACCCAAGCGGGTTCAAGCTTGTG |
| Internal bar code: | AGCCACGTTAGCCAAAGTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 98.5185 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTTTTCCTTCTTAAGGG |
| Suggested primer 2: | TGGTTAGCGGGAGTTTGTTC |