Insertion junction: LMJ.RY0402.190920_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.190920
Chromosome:chromosome 4
Location:2975214
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATCAAGAACCGTTATCCGTTGCCACGGA
Internal bar code:CAATGCTCTCGTCCCCGCCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:227
LEAP-Seq percent confirming:94.0404
LEAP-Seq n confirming:931
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGACTTGTCGCCAGCTTATG
Suggested primer 2:AGCGCTAAACCTGACGTGTT