| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.190926 |
| Chromosome: | chromosome 8 |
| Location: | 324037 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358568 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCTGGGCTCCATGTACAAGCATACCTA |
| Internal bar code: | CGAAACCGGCCCGAGTCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 93.9778 |
| LEAP-Seq n confirming: | 593 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATCTTGTGTTGGTCGTG |
| Suggested primer 2: | AACTGCCCCAAACTATGCAC |