Insertion junction: LMJ.RY0402.190939_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.190939
Chromosome:chromosome 3
Location:3188463
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g165300 (1 of 1) PF13350 - Tyrosine phosphatase family (Y_phosphatase3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACTGTCGTGGTACGACCCCTACCCCAT
Internal bar code:GCAAAGCGCCGGATTTCAGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:41
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTAAAATGGTGGGTATCG
Suggested primer 2:GCGTGTGTGTGTGTGTGTGT