Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.190960 |
Chromosome: | chromosome_14 |
Location: | 1881277 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
None |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGCGCCGCCAGTACTACTGGGGGGGGCGT |
Internal bar code: | AGGGTCGATTCGGCTCCTGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 110 |
LEAP-Seq percent confirming: | 93.4641 |
LEAP-Seq n confirming: | 143 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACTCTTTCGGCGTGATCCT |
Suggested primer 2: | CGTTATGTGTGACCCCTGC |