| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.190995 |
| Chromosome: | chromosome 1 |
| Location: | 6118324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043700 | (1 of 1) PTHR13391//PTHR13391:SF0 - TUBULIN-RELATED PROTEIN // PROTEIN MISATO HOMOLOG 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTTGTGCTTCCCTCAATAGTGGTTCCC |
| Internal bar code: | CGTACGGATTCTCCTTGTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 99.1936 |
| LEAP-Seq n confirming: | 123 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTAGCCTCGTTCAGCGT |
| Suggested primer 2: | ACGAGCCTTGTTCTCTCCAA |